Transfection Reagents
Lonza Walkersville Human T Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DharmaFECT 3 Transfection Reagent, 0.75mL
One of four siRNA/microRNA specific formulations, DharmaFECT 3 is a chemically distinct alternative to one-size-fits-all transfection reagents to achieve high-efficiency silencing in more cell types.
Lonza Walkersville P1 Primary Cell 4D-Nucleofector X Kit S
The P1 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL P1 Primary Cell Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
Sigma Aldrich Fine Chemicals Biosciences DOTAP Liposomal Transfection Reagent | 144189-73-1 | MFCD16660441 | 5 x 400 uL
DOTAP Liposomal Transfection Reagent | Purity: 99% | Mol Wt: 774.19 | 144189-73-1 | MFCD16660441 | 5 x 400 uL
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel24 x Single use pipettes
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P3 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessels12 x Single use pipettes
Agilent Technologies XL2-BLUE MRF ULTRACOMP CELLS
XL2-Blue MRF' Ultracompetent Cells deficient in all known E. coli K12 restriction systems. Increase the efficiency of introducing eukaryotic DNA into E. coli, mcrA, mcrCB and mrr mutations, prevent cleavage of methylated DNA.
Expression Systems BestBac 2.0 Δ v-cath/chiA Baculovirus Cotransfection Kit
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
Contains everything you need to perform cotransfections with your transfer vector. Included in the kit are:
• BestBac DNA
• pVL1393-lacZ control vector
• Transfection medium
Expres2 TR transfection reagent
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
Agilent Technologies XL1-BLUE SUPERCOMPETENT CELLS
Cell, Supercompetent, XL1-Blue; Stratagene; For high-efficiency cloning, greater than equal1 x 109 transformant/ug; Preparation of quality plasmid DNA; Blue-white color screening; Contain antibiotic-resistant F episome; Versatile cloning
Takara Bio Xfect™ RNA Transfection Reagent | 1.2mL
Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA, including messenger RNA (mRNA), small interfering RNA (siRNA), and single guide RNA (sgRNA).The protocol is simple, and transfections can be carried out entirely in the presence of serum. Xfect RNA Transfection Reagent creates biodegradable nanoparticles when combined with RNA, resulting in very low cytotoxicity.